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purity

Page 2 of 33 · 788 questions tagged purity.

788 questions · sorted by votes · page 2 of 33

39 votes 5 answers24k views
Is 97.9% a realistic purity ceiling for survodutide given its chain length?

The specifics, since they change the answer: 97.9% · survodutide. I would like the arithmetic checked rather than the conclusion asserted. I have deliberately not…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by orla_ferriter 47k · 21 months ago
38 votes 5 answers75k views
Would you accept 96.4% on a GLP-1 receptor agonist from QST without a content assay?

Setup, so nobody has to ask: 96.4% · a GLP-1 receptor agonist · QST. I would like to set this up properly once, rather than adjust it repeatedly. My budget is real…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by seven_day_half 16k · 2.1 years ago
38 votes 5 answers66k views
How does retatrutide behave on a C18 column compared to mazdutide?

Stated plainly: retatrutide · a C18 column · mazdutide. I suspect the honest answer is that it depends, in which case I would like to know on what. Assume I can…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by eoin_mcgarry 16k · 22 months ago
38 votes 5 answers57k views
Is a 96.8% result from Medutest comparable to 97.9% from Medutest?

What I am working with: 96.8% · Medutest · 97.9% · Medutest. Both of these get recommended confidently by different people, which suggests neither is obviously right.…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by ahmed_zerouali 19k · 2.0 years ago
38 votes 5 answers54k views
What does a 96.4% purity with a date of analysis attached let me conclude?

Conditions: 96.4% · date of analysis. I would like to know the limits of what can be inferred from this. What I am trying to avoid is over-reading a single result,…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by Dr_Idris_Coulibaly 40k · 2.3 years ago
38 votes 5 answers51k views
Is 98.8% a realistic purity ceiling for mazdutide given its chain length?

Setup, so nobody has to ask: 98.8% · mazdutide. The units are where I keep going wrong, so please be explicit about them. I have sanity-checked the order of magnitude…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by carys_meredith 17k · 2.3 years ago
38 votes5 answers51k views
Would you accept 99.4% on orforglipron from SSA without a content assay?

What I have: 99.4% · orforglipron · SSA. This is a planning question. I know what my options are; I do not know how to weigh them. What I want is the minimum viable…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by lukas_sedlacek 17k · 17 months ago
38 votes 5 answers47k views
Would you accept 98.4% on a GLP-1 receptor agonist from HJ without a content assay?

Setup, so nobody has to ask: 98.4% · a GLP-1 receptor agonist · HJ. I am trying to build something sustainable rather than something thorough that I will abandon. I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by fiadh_cronin 14k · 18 months ago
38 votes3 answers24k views
How do I read an impurity table on a tirzepatide certificate against the 97.6% headline?

For reference: tirzepatide · 97.6%. I would like the arithmetic checked rather than the conclusion asserted. I have deliberately not used an online calculator because…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by Dr_Marek_Zielinski 39k · 19 months ago
37 votes 4 answers66k views
What does a 96.4% purity with a chromatogram attached let me conclude?

The particulars: 96.4% · chromatogram. I want to understand what this actually establishes, as opposed to what it is being used to imply. My concern is that I am…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by fibre_or_fragment 12k · 2.0 years ago
37 votes0 answers40k views
How do I reconcile 99.4% from PeptideMeter with 98.2% from the supplier?

The particulars: 99.4% · PeptideMeter · 98.2%. Something has gone wrong and I would like to know how badly before I decide what to do. Nothing else in the setup…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by rae_oyelowo 21k · 2.2 years ago
37 votes 4 answers39k views
Why would Medutest report 96.8% where the supplier certificate says something higher?

What I have: Medutest · 96.8%. Before I write this off, I want to check whether it is a known failure mode. I have the lot number, the certificate and the date, and I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
asked by mala_venkatesh 21k · 2.3 years ago
37 votes0 answers34k views
Is 98.4% a realistic purity ceiling for ecnoglutide given its chain length?

For reference: 98.4% · ecnoglutide. I would like the arithmetic checked rather than the conclusion asserted. I have deliberately not used an online calculator because…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by pk_curve 12k · 17 months ago
37 votes 2 answers29k views
What does 98.2% purity on a wide-pore C18 column actually tell me about a GLP-1 receptor agonist vial?

For reference: 98.2% · a wide-pore C18 column · a GLP-1 receptor agonist. I want to understand what this actually establishes, as opposed to what it is being used to…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by anouk_desmet 18k · 23 months ago
37 votes 5 answers26k views
What does a 99.1% purity with a purity figure attached let me conclude?

Numbers first: 99.1% · purity figure. I can parse the result. I am less sure what it licenses me to conclude. I have deliberately not looked at anyone else’s…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by loss_on_drying 47k · 2.2 years ago
37 votes 5 answers23k views
Is a 98.8% result from PeptideMeter comparable to 97.6% from Janoshik?

The specifics, since they change the answer: 98.8% · PeptideMeter · 97.6% · Janoshik. The comparison I want does not seem to exist anywhere in a form I can evaluate.…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by net_peptide 16k · 16 months ago
37 votes0 answers18k views
Does fibrillation of semaglutide at 30 °C show up as a loss of content or of purity?

The particulars: fibrillation · semaglutide · 30 °C. I would like the mechanism, because I want to be able to reason about the cases nobody has written about. I have…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by g_paskevicius 44k · 23 months ago
37 votes 3 answers16k views
How do I reconcile 97.9% from PeptideMeter with 97.6% from the supplier?

For reference: 97.9% · PeptideMeter · 97.6%. An unexpected observation, and I would like a differential rather than reassurance. The conditions were within what I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by siobhan_deasy 16k · 18 months ago
37 votes 5 answers14k views
How does semaglutide behave on a C4 column compared to mazdutide?

Concretely: semaglutide · a C4 column · mazdutide. I would like the axes of comparison first and the recommendation second. I have tried the first option and it…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by ivo_paunovic 15k · 16 months ago
36 votes 5 answers64k views
What does 99.1% purity on a C4 column actually tell me about a dulaglutide vial?

Conditions: 99.1% · a C4 column · dulaglutide. I would like to know the limits of what can be inferred from this. What I am trying to avoid is over-reading a single…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by tamsin_wray 13k · 2.0 years ago
36 votes5 answers53k views
How do I reconcile 98.4% from VendorInvestigate with 96.4% from the supplier?

Concretely: 98.4% · VendorInvestigate · 96.4%. I have two candidate explanations and no way to distinguish them. The same procedure has worked without incident…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by mira_sundqvist 19k · 21 months ago
36 votes 5 answers49k views
Does fibrillation produce a resolvable peak on a wide-pore C18 column, or does it co-elute?

The specifics, since they change the answer: fibrillation · a wide-pore C18 column. I understand the observation; what I do not understand is the mechanism behind it.…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
asked by zainab_mustafa 16k · 17 months ago
36 votes3 answers47k views
What does 96.4% purity on a C18 column actually tell me about a semaglutide vial?

What I am working with: 96.4% · a C18 column · semaglutide. I have read the primary source rather than the summary, which has left me with more questions. I…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by plate_count_9k 95k · 2.2 years ago
36 votes 5 answers46k views
What does a 98.8% purity with a purity figure attached let me conclude?

Numbers first: 98.8% · purity figure. I can parse the result. I am less sure what it licenses me to conclude. I have deliberately not looked at anyone else’s…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by colm_dunphy 16k · 15 months ago