PeptideStack
5.2kquestions
20kanswers
220users

injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver the volume you measured.

90 questions · sorted by votes · page 1 of 4

52 votes 3 answers35k views
Red, raised and itchy at the injection site for four days. How do I tell a local reaction from an infection?

Four days ago I injected into the left side of my abdomen. Within an hour there was a coin-sized red area, slightly raised, and it itched. It is now day four: the…

injection-site-reaction
injection-site-reaction

Local reactions: erythema, induration, pruritus and nodules. Their relationship to injection depth, diluent composition, benzyl alcohol…

14 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
subcutaneous
subcutaneous

Subcutaneous administration as a route: absorption kinetics from abdomen versus thigh versus upper arm, tissue depth, and why site rotation…

14 questions
sterility
sterility

Sterility as a test result rather than an adjective. Covers what a sterility test actually measures, why "sterile filtered" on a document is close…

122 questions
asked by linnea_wahlberg 14k · 2.2 years ago
41 votes 5 answers46k views
Is a 31G needle the right choice for drawing survodutide at 8 mg/mL?

The particulars: a 31G needle · survodutide · 8 mg/mL. I am trying to choose between two options that are usually discussed as though only one exists. I am not…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by Dr_Elias_Weiss 46k · 20 months ago
41 votes 5 answers28k views
Is a 31G needle the right choice for drawing ecnoglutide at 2 mg/mL?

The specifics, since they change the answer: a 31G needle · ecnoglutide · 2 mg/mL. The comparison I want does not seem to exist anywhere in a form I can evaluate. I…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by zeynep_arslan 20k · 22 months ago
40 votes0 answers37k views
Is a 27G needle the right choice for drawing a GLP-1 receptor agonist at 4 mg/mL?

Stated plainly: a 27G needle · a GLP-1 receptor agonist · 4 mg/mL. I suspect the honest answer is that it depends, in which case I would like to know on what. Assume…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by jonas_ekstrom 18k · 2.2 years ago
38 votes 5 answers70k views
Should I use a 25G drawing needle to draw and something finer to administer?

For context, I am working with a 10 mg presentation and a 2 mL fill. This is a procedural question rather than a theoretical one, and I would like the procedure…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
sterility
sterility

Sterility as a test result rather than an adjective. Covers what a sterility test actually measures, why "sterile filtered" on a document is close…

122 questions
asked by ahmed_zerouali 19k · 2.0 years ago
38 votes 5 answers41k views
Is a 30G needle the right choice for drawing liraglutide at 1 mg/mL?

Concretely: a 30G needle · liraglutide · 1 mg/mL. I would like the axes of comparison first and the recommendation second. I have tried the first option and it works;…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by m_haraldsen 38k · 22 months ago
37 votes 5 answers34k views
Is a 18G drawing needle the right choice for drawing orforglipron at 20 mg/mL?

Numbers first: an 18G drawing needle · orforglipron · 20 mg/mL. I want to know what the trade-off actually is rather than which option is fashionable. I would rather…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by b_delacroix 48k · 2.0 years ago
37 votes3 answers11k views
Does site rotation actually have evidence behind it, and what kind of reaction warrants stopping?

Two related questions I cannot get straight answers to. First, site rotation. Everyone says rotate, nobody says on what evidence or at what spacing. I have seen "2…

injection-site-reaction
injection-site-reaction

Local reactions: erythema, induration, pruritus and nodules. Their relationship to injection depth, diluent composition, benzyl alcohol…

14 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
subcutaneous
subcutaneous

Subcutaneous administration as a route: absorption kinetics from abdomen versus thigh versus upper arm, tissue depth, and why site rotation…

14 questions
harm-reduction
harm-reduction

Reducing avoidable risk where a decision has already been made: independent verification before use, sterility practice, dose arithmetic checked…

445 questions
asked by klara_novotna 16k · 36 days ago
36 votes5 answers57k views
Is a 30G needle the right choice for drawing cagrilintide at 10 mg/mL?

Stated plainly: a 30G needle · cagrilintide · 10 mg/mL. Both of these get recommended confidently by different people, which suggests neither is obviously right. My…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by esther_vandeVelde 49k · 2.1 years ago
36 votes 5 answers31k views
Is a 30G needle the right choice for drawing a GLP-1 receptor agonist at 6.67 mg/mL?

What I am working with: a 30G needle · a GLP-1 receptor agonist · 6.67 mg/mL. Both of these get recommended confidently by different people, which suggests neither is…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by linnea_wahlberg 14k · 15 months ago
34 votes0 answers13k views
Is a 25G drawing needle the right choice for drawing a GLP-1 receptor agonist at 10 mg/mL?

What I am working with: a 25G drawing needle · a GLP-1 receptor agonist · 10 mg/mL. Both of these get recommended confidently by different people, which suggests…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by birk_nordahl 20k · 22 months ago
33 votes 5 answers35k views
Is a 25G drawing needle the right choice for drawing cagrilintide at 10 mg/mL?

What I am working with: a 25G drawing needle · cagrilintide · 10 mg/mL. I suspect the honest answer is that it depends, in which case I would like to know on what.…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by grainne_ahearn 13k · 13 months ago
33 votes5 answers20k views
Is a 18G drawing needle the right choice for drawing retatrutide at 20 mg/mL?

What I have: an 18G drawing needle · retatrutide · 20 mg/mL. I have used one of these for a while and I am considering switching, which requires a reason. What I care…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by eighty_six_hours 16k · 12 months ago
32 votes5 answers37k views
Is a 18G drawing needle the right choice for drawing retatrutide at 8 mg/mL?

Details up front: an 18G drawing needle · retatrutide · 8 mg/mL. I want to know what the trade-off actually is rather than which option is fashionable. I would rather…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by g_paskevicius 44k · 2.0 years ago
31 votes 5 answers33k views
Is a 30G needle the right choice for drawing tirzepatide at 10 mg/mL?

Conditions: a 30G needle · tirzepatide · 10 mg/mL. These are treated as interchangeable and I do not think they are. If both are acceptable I would like to know that,…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by s_bhattacharya 42k · 12 months ago
31 votes 5 answers30k views
Do I need to let a refrigerated vial come to room temperature before drawing?

I have the same lot stored correctly as a comparator, if a side-by-side test would help. I have done this once and I suspect I got away with it rather than got it…

storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
reconstitution
reconstitution

Taking a lyophilised vial to a solution of known concentration: choice of diluent, volume selection, how to add liquid without shearing the cake,…

330 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
asked by claudia_ferrante 46k · 12 months ago
30 votes5 answers35k views
Is a 30G needle the right choice for drawing oral semaglutide at 10 mg/mL?

Concretely: a 30G needle · oral semaglutide · 10 mg/mL. I would like the axes of comparison first and the recommendation second. I have tried the first option and it…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by sunniva_dahl 11k · 19 months ago
29 votes 5 answers38k views
Should I use a 27G needle to draw and something finer to administer?

I have photographs before and after reconstitution if the visual detail matters. This is a procedural question rather than a theoretical one, and I would like the…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
sterility
sterility

Sterility as a test result rather than an adjective. Covers what a sterility test actually measures, why "sterile filtered" on a document is close…

122 questions
asked by bridget_nyathi 16k · 14 months ago
28 votes5 answers39k views
Is a 31G needle the right choice for drawing ecnoglutide at 20 mg/mL?

For reference: a 31G needle · ecnoglutide · 20 mg/mL. The comparison I want does not seem to exist anywhere in a form I can evaluate. I have read the arguments for…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by area_percent 13k · 21 months ago
28 votes0 answers20k views
Is a 29G needle the right choice for drawing survodutide at 20 mg/mL?

The specifics, since they change the answer: a 29G needle · survodutide · 20 mg/mL. The comparison I want does not seem to exist anywhere in a form I can evaluate. I…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by dana_wexler 15k · 2.3 years ago
28 votes 5 answers12k views
Does injecting into the outer thigh change absorption enough to matter?

I keep a written log of every draw with date, volume and syringe type. I understand the observation; what I do not understand is the mechanism behind it. I have read…

subcutaneous
subcutaneous

Subcutaneous administration as a route: absorption kinetics from abdomen versus thigh versus upper arm, tissue depth, and why site rotation…

14 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
glp1-mechanism
glp1-mechanism

Receptor-level pharmacology: GLP-1R as a class B GPCR, cAMP and PKA signalling, biased agonism, internalisation and resensitisation, and the…

175 questions
asked by anouk_desmet 18k · 14 months ago
25 votes 5 answers39k views
Is a 25G drawing needle the right choice for drawing liraglutide at 10 mg/mL?

The case in front of me: a 25G drawing needle · liraglutide · 10 mg/mL. I would like the axes of comparison first and the recommendation second. I have tried the…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by ruaidhri_o_shea 51k · 19 months ago
25 votes 5 answers21k views
Is a 30G needle the right choice for drawing tirzepatide at 6.67 mg/mL?

The case in front of me: a 30G needle · tirzepatide · 6.67 mg/mL. These are treated as interchangeable and I do not think they are. If both are acceptable I would…

needle-gauge
needle-gauge

Gauge and length selection, the trade-off between draw time and tissue trauma, coring risk with larger-bore needles, and why a fixed-needle…

85 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
dead-space
dead-space

The volume trapped in the syringe hub and needle after the plunger bottoms out. It is small in absolute terms and large as a fraction of a small…

110 questions
asked by gel_pack_warm 13k · 9 months ago
24 votes 5 answers26k views
Do I need to worry about the order I add diluent and swirl?

My setup is a refrigerator with a logger and a small work area I wipe down, nothing more. I have done this once and I suspect I got away with it rather than got it…

reconstitution
reconstitution

Taking a lyophilised vial to a solution of known concentration: choice of diluent, volume selection, how to add liquid without shearing the cake,…

330 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
injection-technique
injection-technique

Technique questions: angle, pinch versus flat, aspiration, injection speed, air bubbles, and the handling steps that determine whether you deliver…

90 questions
asked by cal_hennessy 14k · 18 months ago
Sponsored

Sigma-Aldrich - Certified Reference Materials

Analytical standards and reagents with traceable certificates. Every quantitative result you read inherits the accuracy of the standard behind it.

Shop standards