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peptide-stability

Page 34 of 35 · 832 questions tagged peptide-stability.

832 questions · sorted by votes · page 34 of 35

3 votes5 answers3.9k views
Does liraglutide adsorb measurably to the vial wall at 2.5 mg/mL?

For reference: liraglutide · 2.5 mg/mL. I would rather understand the derivation than memorise the outcome. Two people I asked gave two answers that differ by a…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by birk_nordahl 20k · 8 months ago
3 votes 1 answer3.7k views
Would you re-test mazdutide after sixteen weeks at 2–8 °C, or accept the original certificate?

What I have: mazdutide · sixteen weeks · 2–8 °C. I would rather over-plan the first cycle and simplify later. I am prepared to do the work if someone can tell me…

batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by label_claim 11k · 12 months ago
3 votes 5 answers2.7k views
How long does orforglipron stay within specification at 2–8 °C once reconstituted?

Stated plainly: orforglipron · 2–8 °C. Everything I have found on this is either a forum aside or a product page, neither of which I trust. I am comfortable with the…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by Dr_Lena_Ostrowska 42k · 34 days ago
3 votes0 answers2.3k views
Is there any published stability data for tirzepatide at 40 °C?

The specifics, since they change the answer: tirzepatide · 40 °C. Somebody stated this to me confidently and I would like to check it before repeating it. I would…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
clinical-trials
clinical-trials

Reading the primary literature properly: estimands, intention-to-treat versus per-protocol, confidence intervals, absolute versus relative…

913 questions
asked by ellis_thorne 17k · 16 months ago
3 votes 3 answers2.3k views
Is there any published stability data for semaglutide at 37 °C?

Concretely: semaglutide · 37 °C. The claim is plausible, which is exactly why I want to check it. I am able to read a paper if someone points me at one. Is there data…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
clinical-trials
clinical-trials

Reading the primary literature properly: estimands, intention-to-treat versus per-protocol, confidence intervals, absolute versus relative…

913 questions
asked by swab_and_wait 15k · 44 days ago
3 votes 3 answers2k views
My survodutide vial sat at 40 °C for twenty-one days — is testing worth it before use?

Conditions: survodutide · 40 °C · twenty-one days. The failure mode I am trying to avoid is making this decision emotionally. I have twelve months in view and I would…

cold-chain
cold-chain

Keeping material within a temperature window from manufacture to use: phase-change packs versus dry ice, thermal mass, transit-lane temperature…

524 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
asked by rosa_mendieta 13k · 4 months ago
3 votes 2 answers1.9k views
Is ecnoglutide at 20 mg/mL stable enough for ten weeks of multi-withdrawal use?

The case in front of me: ecnoglutide · 20 mg/mL · ten weeks. The claim is plausible, which is exactly why I want to check it. I am able to read a paper if someone…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
bacteriostatic-water
bacteriostatic-water

Water for injection containing roughly 0.9 per cent benzyl alcohol as a bacteriostatic agent. It suppresses growth in a multiple-withdrawal vial;…

112 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by fresh_bac 13k · 2 months ago
3 votes0 answers1.6k views
What happens to cagrilintide after sixteen weeks at 37 °C in solution?

Details up front: cagrilintide · sixteen weeks · 37 °C. I suspect the usual explanation for this is wrong, or at least incomplete. I am aware this may have a boring…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by g_paskevicius 44k · 11 months ago
3 votes2 answers1.2k views
Does reconstituting retatrutide with phosphate-buffered diluent change anything I should know about?

What I am working with: retatrutide · phosphate-buffered diluent. The empirical answer seems settled. The explanation does not. If the honest answer is that nobody…

reconstitution
reconstitution

Taking a lyophilised vial to a solution of known concentration: choice of diluent, volume selection, how to add liquid without shearing the cake,…

330 questions
bacteriostatic-water
bacteriostatic-water

Water for injection containing roughly 0.9 per cent benzyl alcohol as a bacteriostatic agent. It suppresses growth in a multiple-withdrawal vial;…

112 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
asked by claudia_ferrante 46k · 34 days ago
2 votes5 answers16k views
Would you re-test survodutide after four weeks at 37 °C, or accept the original certificate?

Conditions: survodutide · four weeks · 37 °C. The failure mode I am trying to avoid is making this decision emotionally. I have twelve months in view and I would like…

batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by gradient_slope 41k · 21 months ago
2 votes 3 answers14k views
What happens to ecnoglutide after twelve weeks at 2–8 °C in solution?

Concretely: ecnoglutide · twelve weeks · 2–8 °C. This is one of those things that everyone repeats and nobody derives. This matters practically, not just…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by yuki_morishita 19k · 23 months ago
2 votes 5 answers13k views
Does aggregation of a GLP-1 receptor agonist at room temperature show up as a loss of content or of purity?

Setup, so nobody has to ask: aggregation · a GLP-1 receptor agonist · room temperature. The empirical answer seems settled. The explanation does not. If the honest…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by fibre_or_fragment 12k · 19 months ago
2 votes 5 answers12k views
What happens to ecnoglutide after twelve weeks at 4 °C in solution?

The case in front of me: ecnoglutide · twelve weeks · 4 °C. This is one of those things that everyone repeats and nobody derives. This matters practically, not just…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by harriet_lonsdale 14k · 13 months ago
2 votes5 answers12k views
What happens to orforglipron after two weeks at 37 °C in solution?

What I am working with: orforglipron · two weeks · 37 °C. The empirical answer seems settled. The explanation does not. If the honest answer is that nobody knows, I…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by plunger_stop 18k · 21 months ago
2 votes 4 answers12k views
What happens to orforglipron after two weeks at 4 °C in solution?

Stated plainly: orforglipron · two weeks · 4 °C. The empirical answer seems settled. The explanation does not. If the honest answer is that nobody knows, I would…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by dana_wexler 15k · 13 months ago
2 votes5 answers11k views
Why does aspartimide formation accelerate at minus 80 °C rather than proceeding linearly?

The case in front of me: aspartimide formation · minus 80 °C. I want to know whether this is a real physical effect or an artefact of how it is measured. What…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by loss_on_drying 47k · 22 months ago
2 votes 5 answers9.7k views
Does aggregation of cagrilintide at 25 °C show up as a loss of content or of purity?

Setup, so nobody has to ask: aggregation · cagrilintide · 25 °C. The empirical answer seems settled. The explanation does not. If the honest answer is that nobody…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by nominal_ten 14k · 20 months ago
2 votes 4 answers7.7k views
Does dimerisation of cagrilintide at 25 °C show up as a loss of content or of purity?

What I am working with: dimerisation · cagrilintide · 25 °C. I keep seeing this stated as a fact with no explanation attached, and unexplained facts make me…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by lipid_panel_q 44k · 19 months ago
2 votes 5 answers7.5k views
Would you re-test cagrilintide after six weeks at 2–8 °C, or accept the original certificate?

What I have: cagrilintide · six weeks · 2–8 °C. This is a planning question. I know what my options are; I do not know how to weigh them. What I want is the minimum…

batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
residual-solvent
residual-solvent

Solvents left behind by synthesis and purification - acetonitrile, TFA, DMF, DCM - their ICH limits, how they are measured by headspace GC, and…

14 questions
asked by teodora_ilic 15k · 5 months ago
2 votes 5 answers7.3k views
How many freeze-thaw cycles can a reconstituted solution survive?

I have the same lot stored correctly as a comparator, if a side-by-side test would help. I have done this once and I suspect I got away with it rather than got it…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by seven_day_half 16k · 2.0 years ago
2 votes 5 answers4.7k views
Is 37 °C an acceptable storage condition for lyophilised ecnoglutide?

The particulars: 37 °C · ecnoglutide. Somebody stated this to me confidently and I would like to check it before repeating it. I would accept a well-reasoned negative…

storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by jana_horakova 15k · 2 months ago
2 votes3 answers4.4k views
Would you re-test semaglutide after twelve weeks at 2–8 °C, or accept the original certificate?

Concretely: semaglutide · twelve weeks · 2–8 °C. I want to decide this in advance so that I am not deciding it under pressure later. Assume I will follow the plan I…

batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by dmitri_savchuk 17k · 2.1 years ago
2 votes 5 answers4.3k views
Is minus 80 °C an acceptable storage condition for lyophilised mazdutide?

Setup, so nobody has to ask: minus 80 °C · mazdutide. This has the shape of a fact but I cannot find its origin. What I found instead were three secondary sources all…

storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by fresh_bac 13k · 11 months ago
2 votes 3 answers4k views
Is minus 80 °C an acceptable storage condition for lyophilised ecnoglutide?

For reference: minus 80 °C · ecnoglutide. I would like to know whether this claim survives contact with evidence. If the answer is "nobody has tested that", I would…

storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
shelf-life
shelf-life

How long a preparation remains within specification: labelled expiry for a sealed lyophilised vial, beyond-use dating after reconstitution, and…

301 questions
asked by swab_and_wait 15k · 4 months ago