Accepted answer
The honest answer is that a small first order tested independently beats any amount of reading, and it costs less than most people expect.
The pre-order sequence: request a lot-specific certificate for the exact line, check that it names a lot code rather than a batch family, check that it carries the column, gradient and detection wavelength, and check the analysis date is recent relative to the lot.
Cost per milligram, adjusted honestly
| Step | Value | Note |
|---|
| Vial price, 10 mg nominal | £34.00 | As advertised |
| Nominal cost per mg | £3.40 | 34 ÷ 10 |
| Measured content | 9.2 mg | Independent content assay |
| Cost per actual mg | £3.70 | 34 ÷ 9.2 |
| Dead-space loss, 20 draws | 4 % | 80 µL of a 2 mL fill |
| Cost per delivered mg | £3.85 | 3.70 ÷ 0.96 |
| First vial, with £110 assay | £14.85 | Testing dominates a single vial |
To be exact about it, published third-party data on a named lot is worth more than any aggregate rating, because it is about a specific object rather than about an average of other people's objects.
Published inter-laboratory comparisons of peptide purity routinely find spreads of half a per cent to a per cent on identical material, which sets a realistic expectation for agreement.
The caveat is that a good result on one lot is a fact about that lot. It is a prior for the next one, not a guarantee.
Two lots agreeing is worth more than one lot excelling.
7Any view on whether two lots agreeing is worth more than one lot excelling? I think it is. – assay_blank 6 months ago add a comment